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    Question: If reagent is very small in size, what am I going to do?
    
    Answer: If reagent is very small in size, please centrifuge the reagent bottle before use, to ensure that the reagent concentrate in the bottle.
    
    Question: Do not save reagent as specified?
    
    Answer: not recommended, or reagent quality cannot be guaranteed.
    
    Question: can I mix different lots of the same reagent or kit ?
    
    Answer: you can't.
    
    Question: if wash or not before using coated plates?
    
    Answer: not necessary, but is recommended, because if you omitted this step, it may reduce accuracy.
    
    Question: Can we lengthen or shorten the incubation time?
    
    Answer: the incubation times must be strictly in accordance with the instruction, to get the best results.
    
    Question: must repeat it?
    
    Answer: no need to, but for complex holes of each sample and standard recommendations.
    
    Question: your ELISA Kit can detect which type specimens?
    
    Answer: most of our ELISA kit are applicable in human serum, plasma, cell culture supernatant and other body fluids.Product brochures and on our directory, you will see the specific scope.
    
    Question: did your company standards and international standards-related?
    
    Answer: our following products associated with international standard 1:1
    
    human IFN-γ Int. Referenz NIBSC 82/587 50pg = 1IU?
    
    human IL-2 Int. Referenz NIBSC 86/504 76pg = 1IU?
    
    human IL-5 Int. Referenz NIBSC 90/586 100pg = 1IU?
    
    human IL-6 Int. Referenz NIBSC 89/548 10pg = 1IU?
    
    human IL-10 Int. Referenz NIBSC 93/722 200pg = 1IU?
    
    human IL-12 Int. Referenz NIBSC 95/544 100pg = 1IU?
    
    human IL-13 Int. Referenz NIBSC 94/622 1ng = 1IU?
    
    human TNF-β Int. Referenz NIBSC 87/640 6.7pg = 1IU?
    
    murine IL-2 Int. Referenz NIBSC 93/566 10pg = 1IU?
    
    murine IL-4 Int. Referenz NIBSC 91/656 100pg = 1IU?
    
    murine IL-6 Int. Referenz NIBSC 93/730 10pg = 1IU?
    
    murine IL-10 Int. Referenz NIBSC 93/672 1pg = 1IU?
    
    Problem: high background color is what?
    
    Answer: the background color is too high for the following main reasons:
    
    1) incorrect washing not fully or omit any step in the wash step will cause the background color too much.
    
    Solution: check the volume of wash buffer and make sure to complete all steps of washing;Ensure proper dilution of wash buffer and use it correctly.
    
    2) substrate was polluted
    
    Ensure before use, substrate is not polluted by Metal ions and oxidants; In the experiments, you should keep a few extra substrat; Substrate itself should not have color
    
    3) dilution was polluted
    
    Most of our diluting fluid contains protein, when you use it for the first time, it is likely to cause pollution.When remove the liquid from the bottle,it must use "sterilization" spear head
    
    4) substrates are exposed
    
    Exposure of substrates will turn the substrate into the blue, so before joining plates, you should save it in the shadows (the reagent bottle)
    
    5) The conjugates are diluted by error
    
    The Concentration of conjugates too high can cause high background color, therefore it must dilute in strict accordance with the product instructions.
    
    6) errors of the incubation time and temperature
    
    You Must be strictly in accordance with the product specification to determine the incubation time and temperature
    
    7) Coated plates being saved incorrectly
    
    If the package is only used a part, be sure to correctly save the remaining slats.
    
    Problem: why is the CV (coefficient of variation) too high ?
    
    Answer: it is too high for several reasons:
    
    1) when you wash or draw liquid, scraping into the plate hole created internally coated with antibodies fall off
    
    Note washing coated plates or move standard samples don't scratch the inner surface of the plate.
    
    2) at the time of stripping coated Board cover, it may cause cross-contamination between the holes.
    
    When the incubation is complete, be sure to carefully Strip coated Board cover to avoid cross-contamination between the holes.
    
    3) Samples are not uniform
    
    Ensure that the test sample is homogeneous before joining coated plate to mix.
    
    4) edge effects
    
    You should seal the package at the time of incubation, and incubate at 100 rpm on the oscillator.
    
    5) pipette is not properly calibrated
    
    6) ensure that the pipette is calibrated correctly in the experiment
    
    Problem: why is the standard curve wrong ?
    
    Answer: the following main reasons:
    
    1) Resuspend of the lyophilized standards is incorrect
    
    Ensure that the suspended of lyophilized standard in the right liquid, with the correct size and resuspend. volume of the Resuspend is on both the instructions and on the label indicating of reagent bottle.
    
    2) hang time too short
    
    ensure enough time to completely dissolve freeze dried standard
    
    3) standard is incorrect diluted
    
    standards must be strictly diluted in accordance with the product instructions correctly.
    
    4) standard products and diluent are not saved correctly
    
    the storage of standard product and diluent are listed in the Instruction, you  must be strictly in accordance with the instruction.
    
    5) standard is polluted
    
    Use sterile tips, the right way of saving can prevent pollution.
    
    6) standard dilution is not properly mixed
    
    ensure that each dilution is mixed correctly when the standard is diluted
    
    7) wavelength of the detection is incorrect
    
    ensure the wavelength of Spectrophotometric agent is correct.
    
    8) using other batches of standard
    
    Can only using the same standard in the same Kit, other standard in other batch of the Kit cannot be used.
    
    Question: why does it have color?
    
    Answer:for the following main reasons:
    
    1) restore the reagents to room temperature before the test.
    
    In the experiment to restore all reagents to room temperature before you start.
    
    2) some reagents are not saved correctly
    
    The saving methods of all reagents are detailed in the manual, must be strictly in accordance with its operation.
    
    3) HRP is polluted
    
    ensure there is no contamination Before this reagent is used.
    
    4) dilution of HRP is incorrect
    
    If HRP is diluted excessively, the color will weaken, so you must dilute it according to manual operation.
    
    5) Two kinds of TMB are mixed incorrectly
    
    ensure the correct mixing as shown in the instruction
    
    6) omitting steps of the incubation
    
    any step incubation steps in the Instructions cannot be omitted.
    
    7) plate is drying after washing or in the storage
    
    After washing, Pat plank on the absorbent paper to remove excess wash buffer, immediately after the back steps or on blotting paper of no more than 15 minutes to avoid plate dry.
    
    8) when you wash or move liquid, scraping into the plate hole, so it created antibodies falling off
    
    don't scratch the inner surface of the plate when you wash coated plates or move standard samples.
    
    9) samples are not suitable for this test
    
    Application of specimen for each product are listed in the manual range, out of range of the specimen may not be available in this kit or test conditions. So you  need to fumble (such as control dilution, etc)
    
    Problem: why is the sample or standard "overflow" ?
    
    Answer: it has the following main reasons:
    
    1) sample/standard is incorrectly diluted
    
    It need strictly accordance with the instructions to dilute the samples and standards.
    
    2) coupling reagents are diluted incorrectly
    
    Diluting the coupling reagents must be strictly in accordance with instructions,  too high concentrations will cause OD "overflow"
    
    3) use wrong dilutions
    
    You can only use diluent in the appropriate Kit to diluted sample and standard.
    
    4) The wavelength of detection is incorrect
    
    ensure the wavelength of Spectrophotometric agent is correct
    
    5) The Chromogenic time of TMB is too long
    
    Please monitor Chromogenic time. Color time shown in the instructions may differ slightly in temperature and other conditions change
    
    6) samples are not suitable for this test
    
    Application of the specimen for each product are listed in the manual range, out of range of the specimen may not be available in this kit or test conditions, so it need to fumble (such as control dilution, etc)
    
    7) sample/standard is polluted
    
    The pollution of the sample or standard could lead to results "overflow".
    
    8) The time of incubation /temperature is incorrect
    
    You should ensure the time of incubation and temperature strictly according to the instructions.
    
    9) Cover the Elisa plate cover at the time of incubation
    
    You should cover the Elisa plate cover at the time of incubation in order to prevent evaporation of the liquid or the pollution in the incubation period. Each kit has a sufficient number of Elisa plate cover.
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